Organization and Localization of Septins Changes After Autophagy Induction in Yeast
by Bukre Coskun, Cell Biology ‘18 Authors Note: I wrote this mini journal article for my MCB 140L class. The class focused on the role and structure of septins, a group of proteins that are associated with several biological pathways, like autophagy. I chose to write about a set of experiments we did using yeast-two hybrid assays and fluorescence microscopy to understand the organization and interactions of septins. Abstract: Autophagy is a degradation pathway in which autophagosomes, vesicular structures, deliver proteins and damaged organelles to the lysosomes for degradation. Septins are GTP-binding proteins that localize at the bud-neck and are involved in cytokinesis in budding yeast. Septins also localize at pre-autophagosomal structures and the autophagosome in the autophagy pathway. […]