In Drosophila melanogaster, the highest number of novel gene expression phenotypes have been observed in male reproductive tract tissues. Recent discovery of  a novel testis expression by a gene, CG14662, in D. melanogaster but not sister species suggests a  possible newly-evolved association with male fecundity. This study examined whether CG14662 is associated with reduced male fertility in D. melanogaster. with the use of RNAi knockdown.